Publikationsdatenbank

Expression of VIM, TPI and MAT2A and their impact on in vitro angiogenesis (2024)

Art

Poster

Autoren

Herre, Christina (WE 1)

Nshdejan, Arpenik (WE 1)

Klopfleisch, Robert (WE 12)

Corte, Giuliano Mario

Bahramsoltani, Mahtab (WE 1)

Forschungsprojekt

Charakterisierung endothelialer Progenitorzellen im Prozess der ovariellen zyklischen Neovaskularisation

Kongress

12th Meeting of the Young Generation of Veterinary Anatomists (YGVA)

Zagreb, Kroatien, 17. – 19.07.2024

Quelle

Anatomia, histologia, embryologia

Bandzählung: 53

Heftzählung: S1

Seiten: 14

ISSN: 0340-2096

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/epdf/10.1111/ahe.13066

DOI: 10.1111/ahe.13066

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Angiogenesis is involved in several physiological and pathological events propelling major research efforts since decades. Currently, main focus lies in the field of regenerative medicine. However, the evaluation of in vitro angiogenesis still faces problems regarding reproducibility of results, presumably based on the heterogeneity of endothelial cells (ECs). Being categorized based on their angiogenic potency, vimentin (VIM) and triosephosphate isomerase (TPI) were exclusively found in angiogenic ECs and adenosylmethionine synthetase isoform type-2 (MAT2A) only in non-angiogenic ECs. This study examines the expression of those mentioned proteins and their relation to in vitro angiogenesis in human dermal microvascular endothelial cells (HDMECs). Therefore, two batches of HDMECs were long-term cultivated. Stimulation and quantification of angiogenesis was carried out according to the recently established all-in-one assay (2). A shRNA-mediated knockdown of VIM and TPI was performed separately. The mRNA expression of vascular endothelial growth factor receptor 1 (VEGFR-1) and 2 (VEGFR-2) and mRNA and protein expression of VIM, TPI and MAT2A were determined via RT-qPCR and Western Blot. VIM expression was highest in beginning stages, TPI ascendingly throughout and MAT2A in earliest and late stages of in vitro angiogenesis. VIM and TPI knockdown resulted in a deceleration of angiogenesis, respectively. Knockdown cells with higher VGEFR-1 expression displayed smaller decrease in cell density. Concluding that VIM expression seems to be important for angiogenic stages of sprouting and migration, TPI for most angiogenic stages and MAT2A for quiescence of cells. Knockdowns lead to the suggestion of VIM and TPI being essential proteins and having pro-angiogenic effects on ECs. Furthermore, cell population possessing higher proliferative power was shown to be more resistant to manipulation.

[1] Bahramsoltani M, Harms T, Drewes B, Plendl J (2013) Searching for markers to identify angiogenic endothelial cells: a proteomic approach, Clinical Hemorheology and Microcirculation:55, 255-69.
[2] Bahramsoltani M, De Spiegelaere W (2016). Quantitation of tumor angiogenesis in vitro: An all-in-one angiogenesis assay, Methods in Molecular Biology 1464: 185-191.