Publikationsdatenbank

Cj0683 is a competence protein essential for efficient initialization of DNA uptake in Campylobacter jejuni (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Golz, Julia C.

Preuß, Sandra

Püning, Christoph (WE 8)

Gölz, Greta (WE 8)

Stingl, Kerstin

Forschungsprojekt

PAC-CAMPY - IP2: Biofilme und Strategien zu deren Reduktion

Quelle

Biomolecules : open access journal

Bandzählung: 13

Heftzählung: 3

Seiten: Artikel 514

ISSN: 2218-273x

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2218-273X/13/3/514

DOI: 10.3390/biom13030514

Pubmed: 36979449

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

C. jejuni is an important food-borne pathogen displaying high genetic diversity, substantially based on natural transformation. The mechanism of DNA uptake from the environment depends on a type II secretion/type IV pilus system, whose components are partially known. Here, we quantified DNA uptake in C. jejuni at the single cell level and observed median transport capacities of approximately 30 kb per uptake location. The process appeared to be limited by the initialization of DNA uptake, was finite, and, finalized within 30 min of contact to DNA. Mutants lacking either the outer membrane pore PilQ or the inner membrane channel ComEC were deficient in natural transformation. The periplasmic DNA binding protein ComE was negligible for DNA uptake, which is in contrast to its proposed function. Intriguingly, a mutant lacking the unique periplasmic protein Cj0683 displayed rare but fully functional DNA uptake events. We conclude that Cj0683 was essential for the efficient initialization of DNA uptake, consistent with the putative function as a competence pilus protein. Unravelling features important in natural transformation might lead to target identification, reducing the adaptive potential of pathogens.