Publication Database

Importance of cortactin for efficient epithelial NF-ĸB activation by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa, but not Campylobacter spp. (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Tegtmeyer, Nicole

Soltan Esmaeili, Delara

Sharafutdinov, Irshad

Knorr, Jakob

Naumann, Michael

Alter, Thomas (WE 8)

Backert, Steffen

Forschungsprojekt

PAC-CAMPY - IP2: Biofilme und Strategien zu deren Reduktion

Quelle

European journal of microbiology & immunology

Bandzählung: 11.2021(2022)

Heftzählung: 4

Seiten: 95 – 103

ISSN: 2062-509x

Sprache

Englisch

Verweise

URL (Volltext): https://akjournals.com/view/journals/1886/11/4/article-p95.xml

DOI: 10.1556/1886.2021.00023

Pubmed: 35060920

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Transcription factors of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-ĸB) family control important signaling pathways in the regulation of the host innate immune system. Various bacterial pathogens in the human gastrointestinal tract induce NF-ĸB activity and provoke pro-inflammatory signaling events in infected epithelial cells. NF-ĸB activation requires the phosphorylation-dependent proteolysis of inhibitor of ĸB (IĸB) molecules including the NF-ĸB precursors through ubiquitin-mediated proteolysis. The canonical NF-ĸB pathway merges on IĸB kinases (IKKs), which are required for signal transduction. Using CRISPR-Cas9 technology, secreted embryonic alkaline phosphatase (SEAP) reporter assays and cytokine enzyme-linked immunosorbent assay (ELISA), we demonstrate that the actin-binding protein cortactin is involved in NF-ĸB activation and subsequent interleukin-8 (IL-8) production upon infection by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa. Our data indicate that cortactin is needed to efficiently activate the c-Sarcoma (Src) kinase, which can positively stimulate NF-ĸB during infection. In contrast, cortactin is not involved in activation of NF-ĸB and IL-8 expression upon infection with Campylobacter species C. jejuni, C. coli or C. consisus, suggesting that Campylobacter species pluralis (spp.) induce a different signaling pathway upstream of cortactin to trigger the innate immune response.