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Vibrio (V.) parahaemolyticus is considered as one of the major causes of foodborne illness worldwide, particularly in Asian countries. Most of the infections caused by V. parahaemolyticus are usually associated with the consumption of raw or undercooked seafood. This study was conducted to determine the quantitative load and antimicrobial resistance of V. parahaemolyticus in retail seafood in Hanoi, Vietnam. A total of 120 seafood samples like marine fish (n = 30), oysters (n = 30), shrimp (n = 30) and squid (n = 30) were purchased from different traditional markets in Hanoi from May to October
2020. Isolation of V. parahaemolyticus was based on ISO/TS 21872-1:2007, and MPNPCR was used for quantification. Disc diffusion method was applied for antimicrobial susceptibility testing. Overall, V. parahaemolyticus was detected in 58.3% (70/120) of the samples. V. parahaemolyticus was most commonly found in shrimp samples with the prevalence of 86.7%, followed by marine fish (53.3%), squid (53.3%) and oysters (40%).
Of the positive samples, 27.1% contained V. parahaemolyticus counts of <2 log10 (MPN/g), 44.3% ranged from 2 to 4 log10(MPN/g) and 28.6% contained >4 log10 (MPN/g). Regarding antimicrobial resistance, V. parahaemolyticus isolates showed the highest rate of resistance against ampicillin (81.4%), followed by ceftazidime (11.4%), sulfamethoxazole/trimethoprim (8.6%), cefotaxime (4.3%) and tetracycline (2.9%).
Multi-resistance was found in 12.9% of the V. parahaemolyticus isolates. The results indicate a high prevalence and density as well as antimicrobial resistance of V. parahaemolyticus isolated from seafood sold in the study area.