Publikationsdatenbank

Identification and characterization of immunodominant proteins from tick tissue extracts inducing a protective immune response against Ixodes ricinus in cattle (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Knorr, Sarah (WE 13)

Reissert-Oppermann, Sophia (WE 13)

Tomás-Cortázar, Julen

Barriales, Diego

Azkargorta, Mikel

Iloro, Ibon

Elortza, Félix

Pinecki-Socias, Sophia (WE 13)

Anguita, Juan

Hovius, Joppe W.

Nijhof, Ard M. (WE 13)

Forschungsprojekt

Anti-tick vaccines to prevent tick-borne diseases in Europe

Quelle

Vaccines : open access journal

Bandzählung: 9

Heftzählung: 6

Seiten: Artikel 636

ISSN: 2076-393x

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-393X/9/6/636/pdf

DOI: 10.3390/vaccines9060636

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Ixodes ricinus is the main vector of tick-borne diseases in Europe. An immunization trial of calves with soluble extracts of I. ricinus salivary glands (SGE) or midgut (ME) previously showed a strong response against subsequent tick challenge, resulting in diminished tick feeding success. Immune sera from these trials were used for the co-immunoprecipitation of tick tissue extracts, followed by LC-MS/MS analyses. This resulted in the identification of 46 immunodominant proteins that were differentially recognized by the serum of immunized calves. Some of these proteins had previously also drawn attention as potential anti-tick vaccine candidates using other approaches. Selected proteins were studied in more detail by measuring their relative expression in tick tissues and RNA interference (RNAi) studies. The strongest RNAi phenotypes were observed for MG6 (A0A147BXB7), a protein containing eight fibronectin type III domains predominantly expressed in tick midgut and ovaries of feeding females, and SG2 (A0A0K8RKT7), a glutathione-S-transferase that was found to be upregulated in all investigated tissues upon feeding. The results demonstrated that co-immunoprecipitation of tick proteins with host immune sera followed by protein identification using LC-MS/MS is a valid approach to identify antigen–antibody interactions, and could be integrated into anti-tick vaccine discovery pipelines.