Publikationsdatenbank

Artificial feeding of all consecutive life stages of Ixodes ricinus (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Militzer, Nina (WE 13)

Bartel, Alexander (WE 16)

Clausen, Peter-Henning (WE 13)

Hoffmann-Köhler, Peggy (WE 13)

Nijhof, Ard M. (WE 13)

Forschungsprojekt

Entwicklung neuer Methoden zur Erforschung der zoonotischen Vektorbiologie von Ixodes ricinus mittels CRISPR-Technologie und künstlicher Schildzeckenfütterung

Quelle

Vaccines : open access journal

Bandzählung: 9

Heftzählung: 4

Seiten: Artikel 385

ISSN: 2076-393x

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-393X/9/4/385

DOI: 10.3390/vaccines9040385

Pubmed: 33919961

Kontakt

Institut für Veterinär-Epidemiologie und Biometrie

Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The hard tick Ixodes ricinus is an obligate hematophagous arthropod and the main vector for several zoonotic diseases. The life cycle of this three-host tick species was completed for the first time in vitro by feeding all consecutive life stages using an artificial tick feeding system (ATFS) on heparinized bovine blood supplemented with glucose, adenosine triphosphate, and gentamicin. Relevant physiological parameters were compared to ticks fed on cattle (in vivo). All in vitro feedings lasted significantly longer and the mean engorgement weight of F0 adults and F1 larvae and nymphs was significantly lower compared to ticks fed in vivo. The proportions of engorged ticks were significantly lower for in vitro fed adults and nymphs as well, but higher for in vitro fed larvae. F1-females fed on blood supplemented with vitamin B had a higher detachment proportion and engorgement weight compared to F1-females fed on blood without vitamin B, suggesting that vitamin B supplementation is essential in the artificial feeding of I. ricinus ticks previously exposed to gentamicin.