Publikationsdatenbank

UV-C LED usage for bacterial decontamination of technical surfaces in food processing (2021)

Art

Vortrag

Autoren

Fleischmann, S. (WE 8)

Opherden, S. (WE 8)

Rotsch, P.

Wiese, G.

Alter, T. (WE 8)

Forschungsprojekt

UVC-LED-Einsatz zur Dekontamination technischer Oberflächen in der Lebensmittelverarbeitung (KONTRA), Teilprojekt 1: Optimierung der Inaktivierungseffizienz von UVC-LEDs unter Praxisbedingungen in der Lebensmittelindustrie

Kongress

Advanced UV for Life and the IUVA Confernece ICULTA 2021

online, 19. – 20.04.2021

Quelle

ICULTA 2021. The 2nd International Conference on UV LED Technologies & Applications April 19 – 20, 2021

— S. 103

Sprache

Englisch

Verweise

URL (Volltext): https://www.iculta.com/program

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Contamination and cross contamination of food with zoonotic pathogens in food-producing companies is a main problem of consumer health protection. Every year, the World Health Organization estimates that approximately 420.000 people die worldwide from infections caused by the consumption of contaminated food. Most infections are associated with pathogenic bacteria of the genera Salmonella, Listeria, Campylobacter, Escherichia (E.) coli and Shigella. Furthermore, the spoilage flora causes worldwide the spoilage of a large share of food. Overall, the prevention of cross contamination via technical contact surfaces is a key element in food processing.
Therefore, the aim of this study was the improvement of hygiene in food production through the development, optimization and establishment of efficient procedures for decontamination of contact surfaces via UV-C LED.
For laboratory tests, thin plates of different technical surface material were inoculated with a defined bacterial concentration and the bacterial count was estimated before and after UV-C treatment. The bacterial decontamination efficiency was tested with and without an organic load to simulate a production-related pollution.
Finally, a reproducible method for detecting the decontamination efficiency via UV-C on technical surfaces was developed and established. A high bacterial (109- 107 CFU/ml) and organic load of 3g bovine serum albumin (BSA) resulted in a protective effect of bacteria against UV-C treatment. Gram-positive bacteria like Staphylococcus, Streptococcus and Listeria monocytogenes are less vulnerable to UV-C. A high contamination dose of 109 CFU/ml with E. coli showed a reduction of up to 6log CFU/ml after UV-C LED treatment with a wavelength of 265nm and an irradiance of ~ 5mW/cm2 for 1sec. After a treatment for 5sec, E. coli was no longer detectable.
Concluding the described results, UV-C LED usage is a very useful tool to increase the hygiene of technical surfaces by reducing the bacterial contamination in food processing. However, a production-related organic contamination reduce the irradiation efficiency dramatically. A combined application comprising UV-C LED and a mechanical cleaning of the technical surfaces is therefore an optimal method.