Publication Database

Differences in the transcriptomic response of Campylobacter coli and Campylobacter lari to heat stress (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Riedel, Carolin (WE 8)

Förstner, Konrad U.

Püning, Christoph (WE 8)

Alter, Thomas (WE 8)

Sharma, Cynthia M.

Gölz, Greta (WE 8)

Forschungsprojekt

PAC-CAMPY - IP2: Biofilme und Strategien zu deren Reduktion

Quelle

Frontiers in microbiology

Bandzählung: 11

Seiten: Article 523

ISSN: 1664-302x

Sprache

Englisch

Verweise

URL (Volltext): https://www.frontiersin.org/articles/10.3389/fmicb.2020.00523/full

DOI: 10.3389/fmicb.2020.00523

Pubmed: 32292399

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Campylobacter spp. are one of the most important food-borne pathogens, which are quite susceptible to environmental or technological stressors compared to other zoonotic bacteria. This might be due to the lack of many stress response mechanisms described in other bacteria. Nevertheless, Campylobacter is able to survive in the environment and food products. Although some aspects of the heat stress response in Campylobacter jejuni are already known, information about the stress response in other Campylobacter species are still scarce. In this study, the stress response of Campylobacter coli and Campylobacter lari to elevated temperatures (46°C) was investigated by survival assays and whole transcriptome analysis. None of the strains survived at 46°C for more than 8 h and approximately 20% of the genes of C. coli RM2228 and C. lari RM2100 were differentially expressed. The transcriptomic profiles showed enhanced gene expression of several chaperones like dnaK, groES, groEL, and clpB in both strains, indicating a general involvement in the heat stress response within the Campylobacter species. However, the pronounced differences in the expression pattern between C. coli and C. lari suggest that stress response mechanisms described for one Campylobacter species might be not necessarily transferable to other Campylobacter species.