Publikationsdatenbank

Campylobacter jejuni genes Cj1492c and Cj1507c are involved in Host Cell Adhesion and Invasion (2019)

Art

Poster

Autoren

Xi, D. (WE 3)

Alter, T. (WE 8)

Einspanier, R. (WE 3)

Gölz, G. (WE 8)

Sharbati, S. (WE 3)

Forschungsprojekt

PAC-CAMPY - IP2: Biofilme und Strategien zu deren Reduktion

Kongress

Zoonoses 2019 - International Symposium on Zoonoses Research

Berlin, 16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 218

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The pathogenicity factors of Campylobacter jejuni have not yet been adequately clarified. This study aimed to determine further C. jejuni invasion and pathogenicity factors based on a random mutagenesis approach. A mutant library of C. jejuni NCTC 11168 was generated. The motility, adhesion, invasion, cytotoxicity, intracellular survival and cytokine response have been investigated in human intestinal epithelial cells in vitro. In comparison with wild type, ::Cj1492c and ::Cj1507c showed around 70-80% relative motility and ::Cj1492c had approximately 3-times enhanced adhesion and invasion rates whereas ::Cj1507c possessed clearly diminished adhesive and invasive capability. Only ::Cj1492c had slower in vitrogrowth rate compared with the parental strain.While 24 h post infection 60% of the intracellular wild type were eradicated, significantly fewer mutants were able to survive. No difference in cytotoxicity and induction of the pro-inflammatory chemokines was determined between both mutants and the wild type.Multiple factors of C. jejuni may be required to mediate optimal adhesion and invasion of host cells. Here we report two poorly characterized C. jejuni genes with impact on host cell adhesion and invasion in a reverse manner, which seem to have only a limited effect on the host cell response to infection.