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Listeria monocytogenes is an important human foodborne pathogen and the causative agent of the rare but severe humanlisteriosis. Listeria species are ubiquitous in the environment and frequently found in raw foods(Allerberger and Huhulescu 2015). Many animals, including pigs, and humans can carry the bacterium without showing clinical symptoms.To examine if slaughterpigs are aprimary source of L.monocytogenesin pork, this study investigates the occurrence of the bacterium in slaughter pigs in northwestern Germany as well as in the slaughtering and processing environment. Fecal and tonsillar samples from 200 fattening pigs from 20 herds immediately after slaughter and environmental samples from the slaughterhouse were qualitatively tested for Listeria spp. We found a very low detection frequency of L.monocytogenes in tonsils (1%, 2/200) and could not isolate any L.monocytogenes in fecal samples (0%, 0/200). Positive results of L.monocytogeneswere found in environmental samples (8%, 6/77).Due to these results, we consider tonsils of slaughter pigs as a reservoir for L.monocytogenes and as a low but existent risk for contamination of meat products. In order to determine the zoonotic potential of the isolates, the ANSES institute, EU reference laboratory for L.monocytogenes, will subtype the identified Listeria spp. isolates.This project is funded by the QS Science Fund.