Publikationsdatenbank

Usage of UV-C treatment for bacterial decontamination on eggshells via mercury vapor lamp and LED technology (2020)

Art

Poster

Autoren

Fleischmann, S. (WE 8)

Opherden, S. (WE 8)

Alter, T. (WE 8)

Rosen, K. (WE 10)

Bielicke, M. (WE 10)

Szabo, I.

Hadziabdic, S.

Gensch, A.

Rotsch, P.

Wiese, G.

Roesler, U. (WE 10)

Forschungsprojekt

Einsatz von UV/UV-LED-Strahlung zur Reduktion von Mikroorganismen auf Eiern (UVegg)

Kongress

6th Joint Conference of the DGHM & VAAM

Leipzig, 08. – 11.03.2020

Quelle

6th Joint Conference of the DGHM & VAAM : 8–11 March 2020 Leipzig : Abstracts — German Society for Hygiene and Microbiology, Association for General and Applied Microbiology (Hrsg.)

Leipzig, 2020 — S. 129–130

Sprache

Englisch

Verweise

URL (Volltext): https://www.dghm-vaam.de/fileadmin/congress/media/dghmvaam2020/druckelemente/DGHMVAAM2020_Abstractband.pdf

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The bacterial contamination of eggshells with food-borne zoonotic agents in the commercial egg production is an important One Health issue. The traditional Ultraviolet-C (UV-C) mercury vapor lamp technology is already used for bacterial decontamination on eggshells in individual packaging lines in Germany. A novel system of UV-C light-emitting diodes (LEDs) poses an alternative providing many ecological and economic advantages. The aim of the study was to develop and establish a UV-C LED light method for the commercial application in egg packaging lines. Therefore, the production parameters from an egg packaging line were determined. Thereafter, the bacterial decontamination efficiency of the UV-C LED light on ESBL-producing Escherichia(E.)coli, Enterococcus(E.)faecium, Campylobacterjejuni, Methicillin-resistant Staphylococcusaureus, and Salmonellaon the eggshell surface with and without organic load, was evaluated and compared to the traditional UV-C light method. For this purpose, the surfaces were inoculated with a defined bacterial concentration and the bacterial count was estimated before and after UV-C treatment. Finally, a reproducible method for detecting the decontamination efficiency via UV-C on eggshells was developed and established. A high organic load of 10g bovine serum albumin (BSA) and 10g yeast extract resulted in a protective effect of Gram-negative bacteria against UV-C treatment of up to 2log CFU/cm2in contrast to no organic load. Gram-positive bacteria are less vulnerable to UV-C and stable in their detection rate with and without BSA. A high contamination dose of 107CFU/cm2with E.colishowed only 1logCFU/cm2reduction after UV-C treatment. In contrast, under a contamination dose ≤ 105CFU/cm2E.coliwas no longer detectable. High contamination dose of E.faeciumof 107CFU/cm2 showed only 0,5log reduction and 1log reduction using contamination doses ≤105CFU/cm2after UV-C treatment. In general, field isolates from egg-producing poultry farms showed an increased UV-C tolerance. The comparability of the mercury vapor lamps and the LED panels is possible if the wavelength and the radiation intensity [W/m2] are similar.