Publikationsdatenbank

Characterization of Arcobacter strains isolated from human stool samples:
results from the prospective German prevalence study Arcopath (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Brückner, Vanessa (WE 8)

Fiebiger, Ulrike

Ignatius, Ralf

Friesen, Johannes

Eisenblätter, Martin

Höck, Marlies

Alter, Thomas (WE 8)

Bereswill, Stefan

Heimesaat, Markus M.

Gölz, Greta (WE 8)

Forschungsprojekt

Verbund Arcobacter: Pathogenes Potential und Bedeutung als Zoonosenerreger (Arcopath)

Quelle

Gut pathogens : the official journal of The International Society for Genomic and Evolutionary Microbiology (ISOGEM)

Bandzählung: 12

Seiten: Article number: 3

ISSN: 1757-4749

Sprache

Englisch

Verweise

URL (Volltext): https://doi.org/10.3389/fmicb.2019.03037

DOI: 10.1186/s13099-019-0344-3

Pubmed: 31921357

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Arcobacter constitute emerging food- and waterborne pathogens causing gastroenteritis in humans, but the underlying mechanisms are only incompletely understood. We therefore characterized Arcobacter isolates derived from human stool samples that had been collected during a prospective prevalence study in Germany in vitro. Thirty-six bacterial isolates belonging to the species A. butzleri (n = 24), A. cryaerophilus (n = 10) and A. lanthieri (n = 2) were genotyped by ERIC-PCR, the presence of 10 putative virulence genes was assessed and cytotoxic effects on the human intestinal cell line HT-29/B6 were analyzed applying the WST-assay.

Genotyping revealed high genetic diversity within the species A. butzleri, A. cryaerophilus and A. lanthieri. Both, A. butzleri and A. lanthieri encoded for a large number of putative virulence genes, while fewer genes were detectable in A. cryaerophilus isolates. Notably, the three cytolethal distending toxin (CDT) genes cdtA, cdtB and cdtC were abundant in both A. lanthieri isolates. Furthermore, all A. butzleri and A. lanthieri, but only one of the A. cryaerophilus isolates exerted cytotoxic effects.

Our study provides evidence for the abundance of putative virulence genes in Arcobacter isolates and prominent cytotoxic effects of A. butzleri and A. lanthieri in vitro. The presence of cdtA, cdtB, cdtC in A. lanthieri points towards CDT secretion as potential mechanism underlying cytotoxicity as opposed to A. butzleri. However, the association of the Arcobacter virulence factors detected and human morbidity should be addressed in future studies.