Publication Database

DNase treatment to reduce Campylobacter - and Pseudomonas-biofilms (2019)

Art

Poster

Autoren

Püning, Christoph (WE 8)

Alter, Thomas (WE 8)

Gölz, Greta (WE 8)

Forschungsprojekt

PAC-CAMPY - IP2: Biofilme und Strategien zu deren Reduktion

Kongress

Zoonoses 2019 - International Symposium on Zoonoses Research

Berlin, 16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 255

Sprache

Englisch

Verweise

URL (Volltext): https://www.vetmed.fu-berlin.de/einrichtungen/institute/we08/forschung/poster/2019_Puening_zoonoses/index.html

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Campylobacter (C.) has the ability to colonize and survive in the microaerobic milieu of existing biofilms, which might lead to cross-contamination along the food chain. The reduction of biofilms with DNases can be a potential application to reduce the risk of cross-contamination. Single species biofilms of C. jejuni and Pseudomonas aeruginosa as well as mixed biofilms of both species were grown in 96 well polystyrene plates in Mueller-Hinton broth for 72 h at 37 °C under microaerobic conditions, before several DNase I (2 U/ml) treatments were investigated: DNase I in water i) without a previous washing step, ii) after a previous washing step or iii) in DNase buffer after a previous washing step. Afterwards the biofilm mass was determined by crystal violet staining and measurement of the absorbance. The treatment with DNase I in water without a previous washing step reduced the biofilm mass of all three biofilms. Even though DNase I treatment in water with a previous washing step reduced the mono-species biofilm of C. jejuni, but neither the Pseudomonas mono-species nor the dual-species biofilm mass was reduced. However, if DNase was applied in buffer, the mass of all three biofilms were reduced, even with a previous washing step before DNase I application.Therefore, despite the knowledge about the bacterial composition of existing biofilms along the food chain, it is important to use defined conditions for the DNase I treatment to efficiently reduce the existing biofilms.