Publikationsdatenbank

Isolation and characterization of Arcobacter strains derived from human stool samples – results from a prospective German prevalence study (Arcopath) (2019)

Art

Poster

Autoren

Brückner, Vanessa (WE 8)

Heimesaat, Markus M.

Fiebiger, Ulrike

Ignatius, Ralf

Friesen, Johannes

Eisenblätter, Martin

Höck, Marlies

Bereswill, Stefan

Alter, Thomas (WE 8)

Gölz, Greta (WE 8)

Forschungsprojekt

Verbund Arcobacter: Pathogenes Potential und Bedeutung als Zoonosenerreger (Arcopath)

Kongress

Zoonoses 2019 - International Symposium on Zoonoses Research

Berlin, 16. – 18.10.2019

Quelle

Sprache

Englisch

Verweise

URL (Volltext): https://www.vetmed.fu-berlin.de/einrichtungen/institute/we08/forschung/poster/2019_brueckner_zoo/index.html

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Puumala orthohantavirus (PUUV) is the aetiological agent of nephropathia epidemica (NE), a mild to moderate form of haemorrhagic fever with renal syndrome, which occurs in Fennoscandia, Central and Western Europe and Russia. In Germany, NE-like disease is notifiable since 2001 and the number of hantavirus casesshowed high oscillations between the years. This is mainly caused by beach mast-driven bank vole population dynamics. However, German PUUV strains have never been isolated in cell culture and characterized. In this study, virus isolation attempts were carried out in the field, inoculating naïve bank vole kidney-derived and Vero E6 cell lines with freshly homogenized lung tissue. As an outcome, three virus strainswere isolated from bank voles (Myodes glareolus) trapped in forests close toOsnabrück in Lower Saxony in 2019. The bank vole-derived strains were identified by PUUV specificreal-time and conventional RT-PCR assays and sequence analysis of the S segment. Phylogenetic analysis of the novel PUUV sequences indicated a clustering within the central European clade of PUUV together with other local PUUV sequences. Growth kinetics were determined in both, VeroE6 and MGN-2-R cells, and virus sequence evolution in both cell lines is currently being investigated.In conclusion, a novel protocol for PUUV isolation from bank vole lung tissue was successfully established.