Publikationsdatenbank

Concurrent Proteomic Fingerprinting and Molecular Analysis Of Cyathostomins (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Bredtmann, Christina Maria (WE 13)

Krücken, Jürgen (WE 13)

Murugaiyan, Jayaseelan (WE 10)

Balard, Alice

Hofer, Heribert (WE 13)

Kuzmina, Tetiana A

von Samson-Himmelstjerna, Georg (WE 13)

Forschungsprojekt

Molekulare und proteomische Charakterisierung von Cyathostominen bei Equiden

Quelle

Proteomics

Bandzählung: 19

Heftzählung: 7

Seiten: e1800290

ISSN: 1615-9853

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/full/10.1002/pmic.201800290

DOI: 10.1002/pmic.201800290

Pubmed: 30786147

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Rapid, cost-effective, efficient and reliable helminth species identification is of considerable importance to understand host-parasite interactions, clinical disease and drug resistance. Cyathostomins (Nematoda: Strongylidae) are considered to be the most important equine parasites, yet research on this group has been hampered by the large number of 50 morphologically differentiated species, their occurrence in mixed infections with up to 15 species and the difficulties associated with conventional identification methods. Here, MALDI-TOF MS, previously successfully applied to identify numerous organisms, was evaluated and compared with conventional and molecular genetic approaches. A simple and robust protocol for protein extraction and subsequent DNA isolation allowing molecular confirmation of proteomic findings was developed, showing that MALDI-TOF MS can discriminate adult stages of the two closely related cyathostomin species Cylicostephanus longibursatus and Cylicostephanus minutus. Intraspecific variability of proteomic profiles within morphospecies demonstrated an identification of morphospecies with an accuracy of close to 100%. In contrast, three genospecies within C. minutus and sex-specific profiles within both morphospecies could not be reliably discriminated using MALDI-TOF MS. In conclusion, MALDI-TOF MS complemented by the novel molecular protocol is a reliable and efficient approach for cyathostomin species identification. This article is protected by copyright. All rights reserved.