Publikationsdatenbank

Host response of Campylobacter jejuni mutants with altered invasion capability (2018)

Art

Poster

Autoren

Xi, D. (WE 3)

Gölz, G. (WE 8)

Alter, T. (WE 8)

Sharbati, S. (WE 3)

Forschungsprojekt

PAC-CAMPY - IP2: Biofilme und Strategien zu deren Reduktion

Kongress

National Symposium on Zoonoses Research 2018

Berlin, 17. – 19.10.2018

Quelle

National Symposium on Zoonoses Research 2018, Joint meeting of the German Research Platform for Zoonoses and the Research Network Zoonotic Diseases, Program and Abstracts

— S. 126

Sprache

Englisch

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives: Campylobacter jejuni belongs to the most important foodborne pathogens causing gastrointestinal infections in humans. However, the pathogenicity factors and human host cell responses related to the infection have not yet been adequately clarified. The aim of this study is to determine further C. jejuni invasion factors as well as the underlying regulation of host cell response to infection.
Materials and methods: The motility as well as the capacity of C. jejuni NCTC11168 mutants to invade human intestinal epithelial cells were investigated in vitro. Further, the expression of various mRNAs and lncRNAs, which might be related to cellular responses such as inflammation and apoptosis, were analyzed by means of RT-qPCR.
Results: One of the mutants had higher invasive and motility capacities while another mutant showed significantly decreased invasion and motility rates compared to the wild type. Expression analysis revealed several important coding and noncoding factors in host response to be dysregulated, e.g. reduced expression of a particular lncRNA that is involved in host cell apoptosis. Also, clear differences in the ability to induce IL6 were observed among the mutants.
Conclusion: The available mutations point out two so far uncharacterized genes of C. jejuni that not only play a role in invasiveness but can also influence the host response during infection.