Fachbereich Veterinärmedizin



    VIM-1 carbapenemase-producing Escherichia coli isolated from retail seafood, Germany 2016 (2017)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Roschanski, Nicole (WE 10)
    Guenther, Sebastian (WE 10)
    Vu, Thi Thu Tra (WE 8)
    Fischer, Jennie
    Semmler, Torsten
    Huehn, Stephan (WE 8)
    Alter, Thomas (WE 8)
    Roesler, Uwe (WE 10)
    Prävalenz von ESBL-bildenden Escherichia coli bei Mastputen zur Quantifizierung der relativen Bedeutung von Mastgeflügel und möglicher diesbezüglicher Eintragsquellen
    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin; 22(43) — S. 1
    ISSN: 1560-7917
    DOI: 10.2807/1560-7917.ES.2017.22.43.17-00032
    Pubmed: 29090680
    Institut für Tier- und Umwelthygiene

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    Abstract / Zusammenfassung

    Carbapenems belong to the group of last resort antibiotics in human medicine. Therefore, the emergence of growing numbers of carbapenemase-producing bacteria in food-producing animals or the environment is worrying and an important concern for the public health sector. In the present study, a set of 45 Enterobacteriaceae isolated from German retail seafood (clams and shrimps), sampled in 2016, were investigated by real-time PCR for the presence of carbapenemase-producing bacteria. One Escherichia coli (ST10), isolated from a Venus clam (Ruditapes philippinarum) harvested in the Mediterranean Sea (Italy), contained the carbapenemase gene blaVIM-1 as part of the variable region of a class I integron. Whole-genome sequencing indicated that the integron was embedded in a Tn3-like transposon that also contained the fluoroquinolone resistance gene qnrS1. Additional resistance genes such as the extended-spectrum beta-lactamase blaSHV-12 and the AmpC gene blaACC-1 were also present in this isolate. Except blaACC-1, all resistance genes were located on an IncY plasmid. These results confirm previous observations that carbapenemase-producing bacteria have reached the food chain and are of increasing concern for public health.