Fachbereich Veterinärmedizin



    Gene expression profiling during heat-shock response of Campylobacter (C.) jejuni, C. coli and C. lari (2013)

    Riedel, Carolin (WE 8)
    Gölz, Greta (WE 8)
    Förstner, Konrad U.
    Sharma, Cynthia M.
    Alter, Thomas (WE 8)
    FBI - ZOO: Untersuchungen zur Tenazität ausgewählter Campylobacter jejuni/coli- und Yersinia enterocolitica-Stämme in Lebensmittelmatrizen (TP 08)
    17th International Workshop on Campylobacter, Helicobacter and Related Organisms, CHRO 2013
    Aberdeen, UK, 15. – 19.09.2013
    URL (Volltext): http://www.vetmed.fu-berlin.de/einrichtungen/institute/we08/poster/2013_riedel/index.html
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029

    Abstract / Zusammenfassung

    Although Campylobacter species lack typical stress response mechanisms and sigma factors, they are able to survive in the environment and overcome the barriers along the food chain. The response of C.jejuni to temperatures above the physiological range is sufficiently characterized. Proteomic analyses and gene expression studies revealed an increased expression of common heat-shock genes and synthesis of corresponding proteins. However survival strategies of C. coli and C. lari are still largely unexplored. This study was conducted to examine the response to heat-shock of these two Campylobacter species in comparison to C. jejuni . First, survival rates at 37 °C, 42 °C, 46 °C and 50 °C were mesured. In our study C. jejuni showed a better fitness compared to isolates of C. coli and C. lari . Gene expression studies by real time qPCR revealed an increased expression of different heat-shock genes (dnaK, dnaJ, grpE, groEL, groES, clpB) in C. jejuni, which correlates with earlier studies. The involvement of these chaperones in the heat-shock response of C. coli could be demonstrated as well. In contrast, our results for C. lari suggest alternative heat-shock response mechanisms, since the analyzed known heat-shock genes did not show an altered expression. RNA-Seq data for C. jejuni, C. coli and C. lari provide an explicit global insight in the diverse transcriptome changes as a consequence of temperature upshift from 37 °C to 46°C.