Publikationsdatenbank

New Developments in Diagnostic Tools-opportunities for Food Safety and Outbreak Investigations (2013)

Art

Vortrag

Autor

Alter, Thomas (WE 8)

Forschungsprojekt

Entwicklung und Validierung innovativer produktionsintegrierter mikrobiologischer Stufenkontrollsysteme in der Fleischerzeugung zur Reduktion von Campylobacter (Teilprojekt 4 im Verbundprojekt InnoStep)

Kongress

10th Year Anniversary of Veterinary Public Health Centre for Asia Pacific

The Imperial Mae Ping Hotel, Chiang Mai, Thailand, 02. – 06.07.2013

Quelle

10th Year Anniversary of Veterinary Public Health Centre for Asia Pacific

Chiang Mai, 2013 — S. 18

ISBN: 978-974-672-799-0

Sprache

Englisch

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In the last years, novel diagnostic methods have more and more supported or replaced classical detection methods in food safety. Especially DNA sequence based methods are applied. With these methods, a fast detection of e.g. foodborne pathogens or GMO is possible. In addition, a quantification of these agents can be achieved. PCR based methods can be used to further characterize microorganisms in food (detection of virulence factors, antimicrobial resistance markers, stress response).Genotyping enables us to carry out outbreak investigations by comparing bacterial strains from different sources or to characterize bacterial populations in complex habitats.
Still, some aspects have to be solved:
- How to distinguish viable from dead bacterial cells esp. in processed food?
- The role of viable but not culturable bacterial cells;
- Standardization of genotyping methods to enable a comparison of strains from different regions;
- Bioinformatical analysis.