Publikationsdatenbank

Multicentre Etest evaluation of in vitro activity of conventional antifungal drugs against European bovine mastitis Prototheca spp. isolates (2012)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Jagielski, Tomasz

Buzzini, Pietro

Lassa, Henryka

Malinowski, Edward

Branda, Eva

Turchetti, Benedetta

Polleichtner, Angela

Roesler, Uwe

Lagneau, Paul-Emile

Marques, Sara

Silva, Eliane

Thompson, Gertrude

Stachowiak, Radosław

Bielecki, Jacek

Forschungsprojekt

Pathogene Pflanzen: Epidemiologie und Virulenzmerkmale von Prototheken humaner und tierischer Herkunft

Quelle

The journal of antimicrobial chemotherapy : JAC

Bandzählung: 67

Heftzählung: 8

Seiten: 1945 – 1947

ISSN: 0305-7453

Sprache

Englisch

Verweise

DOI: 10.1093/jac/dks134

Pubmed: 22523316

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Bovine mammary protothecosis is a serious pathology that entails high economic losses in the dairy industry. The disease, the frequency of which has recently been increasing worldwide, is caused by unicellular, achlorophyllous, yeast-like algae of two species: Prototheca zopfii and Prototheca blaschkeae. The objective of this study was to investigate the in vitro activity of a panel of conventional antifungal drugs against Prototheca spp. isolates.

A total of 144 P. zopfii genotype 2 and P. blaschkeae strains isolated from milk of mastitic cows were subjected to drug susceptibility testing by Etest methodology.

Five out of ten antifungal drugs tested exhibited no activity against Prototheca spp. isolates. The best activity against Prototheca spp. was demonstrated by amphotericin B (MIC₉₀ of 1.5 mg/L). The MICs differed significantly (P < 0.01) between P. zopfii genotype 2 and P. blaschkeae, with the latter species being more susceptible to amphotericin B and azoles. Marked differences (P < 0.05) in azole and amphotericin B activities were noted among Prototheca spp. isolates originating from different European countries. Based on the correlation coefficients, a considerable cross-interaction was found among MICs of azoles and between MICs of azoles and amphotericin B for Prototheca spp. (P < 0.03).

This study represents the largest, cross-European evaluation of antifungal activity against Prototheca spp. to date. The activity of amphotericin B against Prototheca spp. validates its potential use as a therapeutic agent against bovine protothecosis. For laboratory testing of drug activity against Prototheca spp., the Etest method is encouraged, due to its technical simplicity, rapidity and high intra- and inter-laboratory reproducibility.