Publikationsdatenbank

Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry (2010)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Dieckmann, R

Strauch, E

Alter, T (WE 8)

Forschungsprojekt

Charakterisierung der Vibrio spp.-Population in Lebensmitteln (C-4); globale Aspekte von Vibrio spp. (C-1); Transkriptom-Profiling von pathogenen Vibrio spp. mittels Microarray-Technik (C6b) im Verbundprojekt VibrioNet

Quelle

Journal of Applied Microbiology

Bandzählung: 109

Heftzählung: 1

Seiten: 199 – 211

ISSN: 1364-5072

Sprache

Englisch

Verweise

DOI: 10.1111/j.1365-2672.2009.04647.x

Pubmed: 20059616

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. This work aimed at evaluating the use of MALDI-TOF mass spectrometry for the rapid identification of Vibrio (V.) spp. as an advantageous application to rapidly discriminate the most important Vibrio spp. and distinguish Vibrio spp. from closely related bacterial species like Photobacterium damselae and Grimontia hollisae and other aquatic bacteria like Aeromonas spp.

Starting from sub-colony amounts of pure cultures grown on agar plates, a very simple sample preparation procedure was established and combined with a rapid and automated measurement protocol that allowed species identification within minutes. Closely related species like Vibrio alginolyticus and Vibrio parahaemolyticus or Vibrio cholerae and Vibrio mimicus could thus be differentiated by defining signatures of species-identifying biomarker ions (SIBIs). As a reference method for species designation and for determination of relationships between strains with molecular markers, partial rpoB gene sequencing was applied.

The MALDI-TOF MS-based method as well as the rpoB sequence-based approach for Vibrio identification described in this study produced comparable classification results. The construction of phylogenetic trees from MALDI-TOF MS and rpoB sequences revealed a very good congruence of both methods.

Our results suggest that whole-cell MALDI-TOF MS-based proteometric characterization represents a powerful tool for rapid and accurate classification and identification of Vibrio spp. and related species.