Publikationsdatenbank

Prion infection of mice transgenic for human APPSwe:
increased accumulation of cortical formic acid extractable Abeta(1-42) and rapid scrapie disease development (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Baier, Michael

Apelt, Jenny

Riemer, Constanze

Gültner, Sandra

Schwarz, Anja

Bamme, Theresa

Burwinkel, Michael

Schliebs, Reinhard

Quelle

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Bandzählung: 26

Heftzählung: 7

Seiten: 821 – 824

ISSN: 0736-5748

Sprache

Englisch

Verweise

DOI: 10.1016/j.ijdevneu.2008.07.001

Pubmed: 18662767

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Neuropathological, epidemiological and experimental data indicate a potential interrelationship between Alzheimer's disease and prion diseases. Proteolytic processing of amyloid precursor protein (APP) by beta-secretase was recently suggested to be controlled by prion protein expression. Here, we characterized the prion infection of Tg2576 mice, which overexpress the human APP(Swe) protein. Prion infection of Tg2576-mice led to an early death of the animals, which was preceded by a relatively short symptomatic stage. However, disease-associated gliosis and deposition of misfolded prion protein PrP(Sc) were identical in infected Tg2576-mice and non-transgenic littermate controls. To analyze the effect of prion infection on APP processing and generation of beta-amyloid we determined cortical levels of SDS- and formic acid (FA)-extractable forms of beta-amyloid (1-40) and (1-42) by ELISA. Formic acid-extractable Abeta (1-42) levels were 10-fold higher in infected versus uninfected Tg2576 mice whereas other forms of Abeta were essentially unaffected by the prion infection. Hence, the experimental model demonstrates that a prion infection of the CNS promotes selectively formation of FA-extractable Abeta(1-42) in Tg2576 mice.