Publikationsdatenbank

S acylation of the hemagglutinin of influenza viruses:
mass spectrometry reveals site-specific attachment of stearic acid to a transmembrane cysteine (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kordyukova, Larisa V

Serebryakova, Marina V

Baratova, Ludmila A

Veit, Michael

Quelle

Journal of virology : publ. by the American Society for Microbiology

Bandzählung: 82

Heftzählung: 18

Seiten: 9288 – 9292

ISSN: 1098-5514

Sprache

Englisch

Verweise

DOI: 10.1128/JVI.00704-08

Pubmed: 18596092

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

S acylation of cysteines located in the transmembrane and/or cytoplasmic region of influenza virus hemagglutinins (HA) contributes to the membrane fusion and assembly of virions. Our results from using mass spectrometry (MS) show that influenza B virus HA possessing two cytoplasmic cysteines contains palmitate, whereas HA-esterase-fusion glycoprotein of influenza C virus having one transmembrane cysteine is stearoylated. HAs of influenza A virus having one transmembrane and two cytoplasmic cysteines contain both palmitate and stearate. MS analysis of recombinant viruses with deletions of individual cysteines, as well as tandem-MS sequencing, revealed the surprising result that stearate is exclusively attached to the cysteine positioned in the transmembrane region of HA.