Publikationsdatenbank

Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Nitschke, Matthias

Korte, Thomas

Tielesch, Claudia

Ter-Avetisyan, Gohar

Tünnemann, Gisela

Cardoso, M Cristina

Veit, Michael

Herrmann, Andreas

Quelle

Virology

Bandzählung: 377

Heftzählung: 2

Seiten: 248 – 254

ISSN: 0042-6822

Sprache

Englisch

Verweise

DOI: 10.1016/j.virol.2008.04.041

Pubmed: 18570963

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.