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    Electron cryomicroscopy reveals different F1+F2 protein States in intact parainfluenza virions (2008)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ludwig, Kai
    Schade, Boris
    Böttcher, Christoph
    Korte, Thomas
    Ohlwein, Nina
    Baljinnyam, Bolormaa
    Veit, Michael
    Herrmann, Andreas
    Quelle
    Journal of virology; 82(7) — S. 3775–3781
    ISSN: 0022-538x
    Sprache
    Englisch
    Verweise
    DOI: 10.1128/JVI.02154-07
    Pubmed: 18216117
    Kontakt
    Institut für Immunologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51834

    Abstract / Zusammenfassung

    Electron cryomicrographs of intact parainfluenza virus 5 (PIV5) virions revealed two different surface structures, namely, a continuous layer and distinct individual spikes. The structure of these spikes reconstructed from intact virions was compared with known F ectodomain structures and was found to be different from the prefusion PIV5 F0 structure but, surprisingly, very similar to the human PIV3 F postfusion structure. Hence, we conclude that the individual F1+F2 spikes in intact PIV5 virions also correspond to the postfusion state. Since the observed fusion activity of PIV5 virions has to be associated with prefusion F1+F2 proteins, they have necessarily to be localized in the continuous surface structure. The data therefore strongly suggest that the prefusion state of the F1+F2 protein requires stabilization, most probably by the association with hemagglutinin-neuraminidase. The conversion of F1+F2 proteins from the prefusion toward the postfusion state while embedded in the virus membrane is topologically difficult to comprehend on the basis of established models and demands reconsideration of our current understanding.