Fachbereich Veterinärmedizin



    Wirkung von Furosemid auf die isolierte normotherm hämoperfundierte Schweineniere : Beitrag zur Validierung eines Modells als Ergänzungs- und Ersatzmethode zum Tierversuch (2001)

    Langenbeck, Nina
    Berlin, 2001 — 115 Seiten
    Institut für Veterinär-Physiologie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62600

    Abstract / Zusammenfassung

    A standardized hemoperfusion was performed on 10 pig kidnevs that were gained during the commercial but modified slaughtering process from pigs with an average weight of 61 kg. The organs were flushed with a .von Baeyer" preservation solution and cooled for 6 hours.During perfusion the arterial pressure was kept under 120 mmHG to avoid organ damage. As a consequence the blood flow rated between 20 to 180 mls per minute which is about 19 to 61 % of the normal blood flow rates given by, literature. The ischaemia led to an acute renal failure and oedema. The glomerular filtration rate and the fraction of sodium reabsorption did not reach physiological levels.As a pharmacological investigation in the isolated perfused kidney frusemide was injected intravenously, into the circuit at a dosage of 1,7 ± 0,35 mg/ 100g kidney weight.The diuresis rose after the frusemide administration (p=0.013), a marked increase in sodium exkretion was seen (p=0.008). Potassium excretion was raised, the fraction of sodium reabsorption was reduced to an average of 72 %. The glomerular filtration rate rose significantly (p=0.038). A rise in oxygen consumption after the frusemide administration was found not to be significant.It could be shown that most of the parameters of kidney function were typically influenced by fursemide (diuresis. sodium excretion, potassium excretion. GM). However, ischemic injuries caused a functional deficiency in the organs which limits the validity of this model.In future the steady state phase will have to be defined more precisely and a higher dosage of frusemide should be used to get more obvious results. It is also worthwhile to use a higher number of kidneys for further investigations and histological examinations.This model can be used to replace animal experiments if the tested parameters are individually controlled. but the reactions of a healthy kideny can not be expected. Therefore the model miht be more useful for research on acute renal failure and organ preservation.