Publikationsdatenbank

Characterization of equine arteritis virus particles and demonstration of their hemolytic activity (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Veit, M

Kabatek, A

Tielesch, C

Hermann, A

Quelle

Archives of virology : official journal of the Virology Division of the International Union of Microbiological Societies

Bandzählung: 153

Heftzählung: 2

Seiten: 351 – 356

ISSN: 0304-8608

Sprache

Englisch

Verweise

DOI: 10.1007/s00705-007-1094-y

Pubmed: 18219439

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Equine arteritis virus (EAV), a member of the newly established family Arteriviridae, is a small, positive-stranded RNA virus. It carries two protein complexes in its envelope, gp5/M and the recently described gp2b/gp3/gp4 complex. We report here on several basic features of EAV replication in cell culture and on the protein composition of virus particles. We have also characterized gp2b, gp3, and gp4 expressed using a baculovirus system in insect cells. Finally, we provide evidence that EAV possess hemagglutinating and hemolytic activity. The hemolysis assay might be useful for determining which of the surface proteins carries the receptor-binding and membrane fusion activity of EAV.