Publikationsdatenbank

The polybasic region is not essential for membrane binding of the matrix protein M1 of influenza virus (2009)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Thaa, Bastian

Herrmann, Andreas

Veit, Michael

Quelle

Virology

Bandzählung: 383

Heftzählung: 1

Seiten: 150 – 155

ISSN: 0042-6822

Sprache

Englisch

Verweise

DOI: 10.1016/j.virol.2008.10.001

Pubmed: 19004463

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The matrix protein M1, the organizer of assembly of influenza virus, interacts with other virus components and with cellular membranes. It has been proposed that M1 binding to lipids is mediated by its polybasic region, but this could hitherto not been investigated in vivo since M1 accumulates in the nucleus of transfected cells. We have equipped M1 with nuclear export signals and showed that the constructs are bound to cellular membranes. Exchange of the complete polybasic region and of further hydrophobic amino acids in its vicinity did not prevent association of M1 with membranes. We therefore suppose that M1 probably interacts with membranes via multiple binding sites.