Publikationsdatenbank

Untersuchung der Hämolyse und Blutzellschädigung von Schweineblut durch verschiedene Blutpumpen im Modell eines künstlichen Kreislaufes (2002)

Art

Hochschulschrift

Autor

Vogel, Peter

Quelle

Berlin: Mensch und Buch Verl, 2002 — 137 Seiten

ISBN: 3-89820-462-6

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Using artificial circuits, isolated organs might be kept vital for several hours. It is beneficial to perfuse the organs using autologous blood. Blood pumps are essential which cause very little or no damage of the blood. Widely used roller pumps do not fulfil this requirement. Hence two new blood pump models were developed and tested. These pumps, named Wechselkammerpumpe and Pulspumpe were compared with three commercially available roller pumps produced by the companies Fresenius, St6ckert and Watson-Marlow. To evaluate the blood damaging effect, free hemoglobin was determined.Free Hemoglobin concentration was standardised, resulting in the calculation of the normalised index of hemolysis (NIH). The NIH of the newly developed pumps was found to be 50-60% lower compared to the roller pumps, proving that the newly developed pumps are suitable for an application in artificial circuits.Further the effects of the pumps on thrombocytes and leukocytes were examined. Here the cell number of these blood cells was determined. The concentration of the thrombocytes took during the attempt period with the ollenpumpen nearly around the double value off, as with the two new blood pump models. The leukocyte numbers showed only slight changes and remained relatively stable with all pumps.Future studies should investigate which constructive and engineering efforts are necessary for an application of the pumps in routine experiments. This work was promoted by the Federal Ministry for education and research within the framework of the program "spare methods for the bioassay".