Publikationsdatenbank

Intrinsic cytoskeleton-dependent clustering of influenza virus M2 protein with hemagglutinin assessed by FLIM-FRET (2010)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Thaa, Bastian

Herrmann, Andreas

Veit, Michael

Quelle

Journal of virology : publ. by the American Society for Microbiology

Bandzählung: 84

Heftzählung: 23

Seiten: 12445 – 12449

ISSN: 1098-5514

Sprache

Englisch

Verweise

DOI: 10.1128/JVI.01322-10

Pubmed: 20881046

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The hemagglutinin (HA) of influenza virus organizes the virus bud zone, a domain of the plasma membrane enriched in raft lipids. Using fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer (FLIM-FRET), a technique that detects close colocalization of fluorescent proteins in transfected cells, we show that the viral proton channel M2 clusters with HA but not with a marker for inner leaflet rafts. The FRET signal between M2 and HA depends on the raft-targeting signals in HA and on an intact actin cytoskeleton. We conclude that M2 contains an intrinsic signal that targets the protein to the viral bud zone, which is organized by raft-associated HA and by cortical actin.