Fachbereich Veterinärmedizin



    Hemagglutinin of influenza virus partitions into the nonraft domain of model membranes (2010)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Nikolaus, Jörg
    Scolari, Silvia
    Bayraktarov, Elisa
    Jungnick, Nadine
    Engel, Stephanie
    Pia Plazzo, Anna
    Stöckl, Martin
    Volkmer, Rudolf
    Veit, Michael
    Herrmann, Andreas
    Biophysical journal; 99(2) — S. 489–498
    ISSN: 0006-3495
    DOI: 10.1016/j.bpj.2010.04.027
    Pubmed: 20643067
    Institut für Immunologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51834

    Abstract / Zusammenfassung

    The HA of influenza virus is a paradigm for a transmembrane protein thought to be associated with membrane-rafts, liquid-ordered like nanodomains of the plasma membrane enriched in cholesterol, glycosphingolipids, and saturated phospholipids. Due to their submicron size in cells, rafts can not be visualized directly and raft-association of HA was hitherto analyzed by indirect methods. In this study, we have used GUVs and GPMVs, showing liquid disordered and liquid ordered domains, to directly visualize partition of HA by fluorescence microscopy. We show that HA is exclusively (GUVs) or predominantly (GPMVs) present in the liquid disordered domain, regardless of whether authentic HA or domains containing its raft targeting signals were reconstituted into model membranes. The preferential partition of HA into ld domains and the difference between lo partition in GUV and GPMV are discussed with respect to differences in packaging of lipids in membranes of model systems and living cells suggesting that physical properties of lipid domains in biological membranes are tightly regulated by protein-lipid interactions.