Publikationsdatenbank

Hemagglutinin of influenza virus partitions into the nonraft domain of model membranes (2010)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Nikolaus, Jörg

Scolari, Silvia

Bayraktarov, Elisa

Jungnick, Nadine

Engel, Stephanie

Pia Plazzo, Anna

Stöckl, Martin

Volkmer, Rudolf

Veit, Michael

Herrmann, Andreas

Quelle

Biophysical journal

Bandzählung: 99

Heftzählung: 2

Seiten: 489 – 498

ISSN: 0006-3495

Sprache

Englisch

Verweise

DOI: 10.1016/j.bpj.2010.04.027

Pubmed: 20643067

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The HA of influenza virus is a paradigm for a transmembrane protein thought to be associated with membrane-rafts, liquid-ordered like nanodomains of the plasma membrane enriched in cholesterol, glycosphingolipids, and saturated phospholipids. Due to their submicron size in cells, rafts can not be visualized directly and raft-association of HA was hitherto analyzed by indirect methods. In this study, we have used GUVs and GPMVs, showing liquid disordered and liquid ordered domains, to directly visualize partition of HA by fluorescence microscopy. We show that HA is exclusively (GUVs) or predominantly (GPMVs) present in the liquid disordered domain, regardless of whether authentic HA or domains containing its raft targeting signals were reconstituted into model membranes. The preferential partition of HA into ld domains and the difference between lo partition in GUV and GPMV are discussed with respect to differences in packaging of lipids in membranes of model systems and living cells suggesting that physical properties of lipid domains in biological membranes are tightly regulated by protein-lipid interactions.