Fachbereich Veterinärmedizin



    A helminth immunomodulator exploits host signaling events to regulate cytokine production in macrophages (2011)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Klotz, Christian
    Ziegler, Thomas
    Figueiredo, Ana Sofia
    Rausch, Sebastian (WE 6)
    Hepworth, Matthew R (WE 6)
    Obsivac, Nadja
    Sers, Christine
    Lang, Roland
    Hammerstein, Peter
    Lucius, Richard
    Hartmann, Susanne (WE 6)
    PLoS Pathogens; 7(1) — S. e1001248
    ISSN: 1553-7366
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000019823
    DOI: 10.1371/journal.ppat.1001248
    Pubmed: 21253577
    Institut für Immunologie und Molekularbiologie

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    Abstract / Zusammenfassung

    Parasitic worms alter their host's immune system to diminish the inflammatory responses directed against them, using very efficient immunomodulating molecules. We have previously shown that the helminth immunomodulator cystatin (AvCystatin) profoundly reduces the progression of inflammatory diseases via modulation of macrophages. Here we elucidate the signaling events in macrophages triggered by AvCystatin. Labeled AvCystatin was predominantly taken up by macrophages and subsequently induced the phosphorylation of the mitogen-activated protein kinases (MAPK) ERK1/2 and p38. IL-10 expression induced by AvCystatin in macrophages was tyrosine kinase sensitive and dependent on activation of both MAP kinases, in clear contrast to expression of IL-12/23p40. In addition, phosphorylation of the transcription factors CREB and STAT3 was induced by AvCystatin and regulated by phospho-ERK. Chemical inhibition of phosphoinositide 3-kinase (PI3K) reduced AvCystatin-induced cytokine release; however, AKT, the downstream target of PI3K, was not activated following AvCystatin exposure. To characterize signaling elements involved in alteration of the macrophage phenotype we applied mathematical modeling. Experimental testing of the in silico generated hypotheses identified dual specificity phosphatase (DUSP) 1 and 2, as regulators in AvCystatin triggered macrophages in vitro and in vivo. In particular, DUSP1 was subsequently found to be responsible for regulation of ERK- and p38-phosphorylation and controlled the IL-10 expression in macrophages by AvCystatin. Thus, we show that AvCystatin exploits activation and deactivation pathways of MAP kinases to induce regulatory macrophages. This study provides insights into molecular mechanisms of macrophage manipulation by parasites and highlights the utility of mathematical modeling for the elucidation of regulatory circuits of immune cells.