Publikationsdatenbank

Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs (2006)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Forterre, Simone

Raila, Jens

Forterre, Franck

Brunnberg, Leo

Schweigert, Florian J

Quelle

The veterinary journal

Bandzählung: 171

Heftzählung: 3

Seiten: 451 – 455

ISSN: 1532-2971

Sprache

Englisch

Verweise

Pubmed: 16624711

Kontakt

Klein- und Heimtierklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62422
kleintierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.