Publikationsdatenbank

Application of laser postionization secondary neutral mass spectrometry/time-of-flight secondary ion mass spectrometry in nanotoxicology: visualization of nanosilver in human macrophages and cellular responses (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Haase, Andrea

Arlinghaus, Heinrich F

Tentschert, Jutta

Jungnickel, Harald

Graf, Philipp

Mantion, Alexandre

Draude, Felix

Galla, Sebastian

Plendl, Johanna (WE 1)

Goetz, Mario E

Masic, Admir

Meier, Wolfgang

Thünemann, Andreas F

Taubert, Andreas

Luch, Andreas

Quelle

ACS nano

Bandzählung: 5

Heftzählung: 4

Seiten: 3059 – 68

ISSN: 1936-0851

Sprache

Englisch

Verweise

DOI: 10.1021/nn200163w

Pubmed: 21456612

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Silver nanoparticles (SNP) are the subject of worldwide commercialization because of their antimicrobial effects. Yet only little data on their mode of action exist. Further, only few techniques allow for visualization and quantification of unlabeled nanoparticles inside cells. To study SNP of different sizes and coatings within human macrophages, we introduce a novel laser postionization secondary neutral mass spectrometry (Laser-SNMS) approach and prove this method superior to the widely applied confocal Raman and transmission electron microscopy. With time-of-flight secondary ion mass spectrometry (TOF-SIMS) we further demonstrate characteristic fingerprints in the lipid pattern of the cellular membrane indicative of oxidative stress and membrane fluidity changes. Increases of protein carbonyl and heme oxygenase-1 levels in treated cells confirm the presence of oxidative stress biochemically. Intriguingly, affected phagocytosis reveals as highly sensitive end point of SNP-mediated adversity in macrophages. The cellular responses monitored are hierarchically linked, but follow individual kinetics and are partially reversible.