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    Uncertainty from sampling in measurements of aflatoxins in animal feedingstuffs:
    application of the Eurachem/CITAC guidelines (2011)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Reiter, Elisabeth Viktoria
    Dutton, Mike Francis
    Agus, Ali
    Nordkvist, Erik
    Mwanza, Mulunda Feza
    Njobeh, Patrick Berka
    Prawano, Deni
    Häggblom, Per
    Razzazi-Fazeli, Ebrahim
    Zentek, Jürgen
    Andersson, Mats Gunnar
    Quelle
    The analyst : the analytical journal of the Royal Society of Chemistry; 136(19) — S. 4059–69
    ISSN: 0003-2654
    Sprache
    Englisch
    Verweise
    DOI: 10.1039/c1an15124j
    Pubmed: 21833409
    Kontakt
    Institut für Tierernährung

    Königin-Luise-Str. 49
    Gebäude 8
    14195 Berlin
    Tel.+49 30 838 52256 Fax.+49 30 838-55938
    email:tierernaehrung@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The duplicate method for estimating uncertainty from measurement including sampling is presented in the Eurachem/CITAC guide. The applicability of this method as a tool for verifying sampling plans for mycotoxins was assessed in three case studies with aflatoxin B(1) in animal feedingstuffs. Aspects considered included strategies for obtaining samples from contaminated lots, assumptions about distributions, approaches for statistical analysis, log(10)-transformation of test data and applicability of uncertainty estimates. The results showed that when duplicate aggregate samples are formed by interpenetrating sampling, repeated measurements from a lot can be assumed to approximately follow a normal or lognormal distribution. Due to the large variation in toxin concentration between sampling targets and sometimes very large uncertainty arising from sampling and sample preparation (U(rel) ≥ 50%), estimation of uncertainty from log(10)-transformed data was found to be a more generally applicable approach than application of robust ANOVA.