Publikationsdatenbank

Uncertainty from sampling in measurements of aflatoxins in animal feedingstuffs:
application of the Eurachem/CITAC guidelines (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Reiter, Elisabeth Viktoria

Dutton, Mike Francis

Agus, Ali

Nordkvist, Erik

Mwanza, Mulunda Feza

Njobeh, Patrick Berka

Prawano, Deni

Häggblom, Per

Razzazi-Fazeli, Ebrahim

Zentek, Jürgen

Andersson, Mats Gunnar

Quelle

The analyst : the analytical journal of the Royal Society of Chemistry

Bandzählung: 136

Heftzählung: 19

Seiten: 4059 – 69

ISSN: 0003-2654

Sprache

Englisch

Verweise

DOI: 10.1039/c1an15124j

Pubmed: 21833409

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The duplicate method for estimating uncertainty from measurement including sampling is presented in the Eurachem/CITAC guide. The applicability of this method as a tool for verifying sampling plans for mycotoxins was assessed in three case studies with aflatoxin B(1) in animal feedingstuffs. Aspects considered included strategies for obtaining samples from contaminated lots, assumptions about distributions, approaches for statistical analysis, log(10)-transformation of test data and applicability of uncertainty estimates. The results showed that when duplicate aggregate samples are formed by interpenetrating sampling, repeated measurements from a lot can be assumed to approximately follow a normal or lognormal distribution. Due to the large variation in toxin concentration between sampling targets and sometimes very large uncertainty arising from sampling and sample preparation (U(rel) ≥ 50%), estimation of uncertainty from log(10)-transformed data was found to be a more generally applicable approach than application of robust ANOVA.