Fachbereich Veterinärmedizin



    Untersuchungen über das Vorkommen von Campylobacter-, Salmonellen- und Verotoxinbildenden E. coli-Keimen in Putenmastbetrieben und in einer Putenschlachtanlage vor und nach Durchführung von Reinigungs- und Desinfektionsmaßnahmen (2001)

    Ruckaberle, Elke Katrin
    Berlin, 2001 — 207 Seiten
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    +49 30 838 62676

    Abstract / Zusammenfassung

    The aim of the present study was to collect data on the prevalence of Campylobacter, Salmonella and VTEC strains in meat turkey farms and in a turkey slaughterhouse and to test the efficacy of current cleansing and disinfection regimens und er field conditions.Therefore faecal samples were taken in ten meat turkey flocks about 14 days before slaughter. Immediately after the birds were taken to slaughterhouse again faecal samples and also dust, litter and environmental samples were examined. After cleansing and disinfection environmental samples from the same places vvere again collected and examined.Before cleansing and disinfection Campylobacter could be detected in 8 out of 10 farms (80%). The bacteria was mostly recovered from faecal samples, litter samples, the floor in the entry of the houses and the waterers. Salmonella could be recovered in 3 out of 10 Farms (30 %). After cleansing and disinfection no Campylobacter was detected whereas the same Salmonella serotype was reisolated in 1 of the 3 positive farms. VTEC or Verotoxins could not be detected in any samples.In the turkey slaughterhouse samples from machines and equipment were taken before and after cleansing and disinfection. The slaughterhouse was tested at two independant times. At both sampling times only Campylobacter could be detected before cleansing and disinfection at 9 out of 12 tested areas. After cleansing and disinfection no Campylobacter strain could be recovered. Salmonella and VTEC or Verotoxins were not found at the examination of the slaughterhouse.The isolated bacteria were checked by pulsed-field-gel-electrophoreses for clonal relatedness. The results revealed that within a poultry house or a poultry slaughterhouse genetically identical and also genetically not identical Campylobacter strains may occur. It was evident that all the Salmonella Anatum isolates from two different farms were genetically related.The Campylobacter and Salmonella strains were also tested for their sensitivity to antimicrobial substances. A high proportion of the Campylobacter strains showed resistance to Tetracycline, Ampicillin, Nalidixic Acid and Fluorochinolones whereas the Salmonella Anatum strains showed multiple drug resistances.