Publikationsdatenbank

Investigations of the Japanese bovine tumour virus (BLV):
its ability to express structural and regulatory BLV proteins (1996)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Blankenstein, P

Bondzio, A

Büchel, A

Kinder, E

Ebner, D

Quelle

Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B

Bandzählung: 43

Heftzählung: 6

Seiten: 343 – 349

ISSN: 0514-7166

Sprache

Englisch

Verweise

Pubmed: 8794696

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The mechanism of BLV-induced tumorigenesis has not been clear up to now. Changes of viral protein expression in infected cells may be involved in the molecular events leading to BLV-induced leukaemogenesis. In this study Western blot investigations of cells transfected with plasmid DNA containing the complete Japanese BLV tumour clone provirus demonstrate that this provirus is unable to express gag and env proteins. Following this an attempt was made to express the genes from this provirus in eukaryotic and prokaryotic cells using the phagemid pBK-RSV (Stratagene), but not as fusion proteins. The protein patterns expressed from the 5' and the 3' region of the BLV genome were compared with those of FLK/BLV cells. The results indicate that there is a defect in this provirus located in the genome region between the gag and env gene.