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    Involvement of intracellular Ca2+ in the regulation of bovine leukemia virus expression (2001)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Bondzio, A
    Abraham-Podgornik, A
    Blankenstein, P
    Risse, S
    Quelle
    Biological chemistry; 382(3) — S. 407–416
    ISSN: 1431-6730
    Sprache
    Englisch
    Verweise
    Pubmed: 11347888
    Kontakt
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    Tel.+49 30 838 62225 Fax.+49 30 838-62584
    email:biochemie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The calcium ionophore A23187, which was used to increase the intracellular calcium concentration ([Ca2+]i), was analyzed for effects on bovine leukemia virus (BLV) expression in two BLV infected cell lines. To clarify the role of intracellular free calcium in this response, [Ca2+]i was measured during ionophore treatment with the fluorescent calcium indicator Fura-2. Elevation of intracellular calcium under these conditions caused an enhancement of BLV gp51 and p24 synthesis as well as an activation of the BLV long terminal repeat (LTR) in a dose-dependent manner. Furthermore, it was observed that elevated levels of intracellular calcium following A23187 stimulation lead to activation of NF-kappaB. Based on inhibitor studies, we hypothesize that the effect of A23187 on BLV expression appears to be mediated by PKC.