Publikationsdatenbank

Involvement of intracellular Ca2+ in the regulation of bovine leukemia virus expression (2001)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Bondzio, A

Abraham-Podgornik, A

Blankenstein, P

Risse, S

Quelle

Biological chemistry

Bandzählung: 382

Heftzählung: 3

Seiten: 407 – 416

ISSN: 1431-6730

Sprache

Englisch

Verweise

Pubmed: 11347888

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The calcium ionophore A23187, which was used to increase the intracellular calcium concentration ([Ca2+]i), was analyzed for effects on bovine leukemia virus (BLV) expression in two BLV infected cell lines. To clarify the role of intracellular free calcium in this response, [Ca2+]i was measured during ionophore treatment with the fluorescent calcium indicator Fura-2. Elevation of intracellular calcium under these conditions caused an enhancement of BLV gp51 and p24 synthesis as well as an activation of the BLV long terminal repeat (LTR) in a dose-dependent manner. Furthermore, it was observed that elevated levels of intracellular calcium following A23187 stimulation lead to activation of NF-kappaB. Based on inhibitor studies, we hypothesize that the effect of A23187 on BLV expression appears to be mediated by PKC.