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    Accumulation of histones in cell lysates precedes expression of apoptosis-related phagocytosis signals in human lymphoblasts (2003)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Gabler, Christoph
    Blank, Norbert
    Winkler, Silke
    Kalden, Joachim R
    Lorenz, Hanns-M
    Quelle
    Annals of the New York Academy of Sciences; 1010 — S. 221–224
    ISSN: 0077-8923
    Sprache
    Englisch
    Verweise
    Pubmed: 15033725
    Kontakt
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    Tel.+49 30 838 62225 Fax.+49 30 838-62584
    email:biochemie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Systemic lupus erythematosus (SLE) is characterized by the production of autoantibodies directed against several nuclear components, such as DNA and histones. Apoptosis was induced in activated human lymphoblasts (n = 6) by UV-B irradiation for 30 sec followed by continuous culturing. An extranuclear accumulation of the nucleosomal histones H2A, H2B, H3, and H4 in cell lysates was observed very early in the process of apoptosis, even before phosphatidylserine externalization occurred on the outer membrane surface of apoptotically dying lymphoblasts. We hypothesize that a dysregulation of apoptosis during these early phases may contribute to the induction of autoimmunity against nuclear autoantigens as seen in SLE.