Publikationsdatenbank

Accumulation of histones in cell lysates precedes expression of apoptosis-related phagocytosis signals in human lymphoblasts (2003)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Gabler, Christoph

Blank, Norbert

Winkler, Silke

Kalden, Joachim R

Lorenz, Hanns-M

Quelle

Annals of the New York Academy of Sciences

Bandzählung: 1010

Seiten: 221 – 224

ISSN: 1749-6632

Sprache

Englisch

Verweise

Pubmed: 15033725

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Systemic lupus erythematosus (SLE) is characterized by the production of autoantibodies directed against several nuclear components, such as DNA and histones. Apoptosis was induced in activated human lymphoblasts (n = 6) by UV-B irradiation for 30 sec followed by continuous culturing. An extranuclear accumulation of the nucleosomal histones H2A, H2B, H3, and H4 in cell lysates was observed very early in the process of apoptosis, even before phosphatidylserine externalization occurred on the outer membrane surface of apoptotically dying lymphoblasts. We hypothesize that a dysregulation of apoptosis during these early phases may contribute to the induction of autoimmunity against nuclear autoantigens as seen in SLE.