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Water and lipid-soluble antioxidative capacities were evaluated from blood samples taken from dairy cows in different lactation stages, and from diseased and healthy dairy cattle.
Blood samples using different media were taken, from dairy cattle in different lacatation stages, diseased, and healthy, in the clinic of cattle, faculty of veterinary medicine, Free university, Berlin. The first step was evaluation of the stability of the water soluble antioxidative capacity in different media used, using the photochem, the Serum samples were choosen because of the high values achieved using these tubes.
After that, the stability of ACW was tested using Serum tubes under different temperatures within a specific period, the samples kept under liquid nitrogen, -24C˚, -20C˚ were stable within 168 hours, and the samples kept under room temperature (+25C˚), and +4C˚ were not stable, the first changes were noticed after 2 hours.
Different postpartum times were tested, the ACW values reflect the internal physiological status of the animals. And animals suffering from stress around delivery have lower values than cows in the other groups. Different farms are also compared
It was not possible to measure the lipid soluble antioxidative capacity, because of a failure in the tubules in the apparatus.