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    Angiopoietins differentially influence in vitro angiogenesis by endothelial cells of different origin (2011)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    de Spiegelaere, Ward
    Cornillie, Pieter
    van den Broeck, Wim
    Plendl, Johanna (WE 1)
    Bahramsoltani, Mahtab
    Quelle
    Clinical hemorheology and microcirculation; 48(1) — S. 15–27
    ISSN: 1386-0291
    Sprache
    Englisch
    Verweise
    DOI: 10.3233/CH-2011-1393
    Pubmed: 21876231
    Kontakt
    Institut für Veterinär-Anatomie

    Koserstr. 20
    14195 Berlin
    +49 30 838 53555
    anatomie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Angiopoietins are important growth factors for vascular development and quiescence. They are promising targets for pro- or anti-angiogenic therapies in diverse pathologies, but the mechanisms of the ANGPT/TIE2 system are complex and not well understood. In the present study, the separate and combined effects of angiopoietin 1 and angiopoietin 2 were studied, using a recently developed in vitro angiogenesis model that allows both a quantitative and qualitative evaluation of the angiogenic cascade. This cell culture model was performed with microvascular endothelial cells (ECs) originating from different vascular beds, i.e. dermal ECs and cardiac ECs. In addition, the expression of the angiopoietins and the receptors, TIE1 and TIE2 was analyzed with RT-qPCR. This study revealed that the angiopoietins provoked a differential response in the two endothelial cultures. Both angiopoietin 1 as well as angiopoietin 2 elicited an angiogenic cascade in the dermal ECs but not in the cardiac ECs. In addition, the RT-qPCR data revealed marked differences in the endogenous expression pattern of these factors, indicating that the origin of endothelial cells might have an important impact on their angiogenic potential.