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    Enzymimmunoassay (ELISA) zum Koproantigennachweis der Monieziose des Schafes (2001)

    Art
    Hochschulschrift
    Autor
    Leser, Karsten
    Quelle
    Berlin, 2001 — 107 Seiten
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    Tel.+49 30 838 62310 Fax.+49 30 838 62323
    email:parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Topic and objective of the submitted paper was to evolve a sandwich enzyme immunoassay (ELISA), which can detect coproantigens of Moniezia in samples of sheep"s feaces.By immunizing rabbits and chicken with somatic antigen-preparations, tapeworm antibodies could be extracted which then were used with the coproantigen-ELISA.Polyclonal anti-cestode-lgG of hyperimmunizated rabbits cleaned with a protein-A column served as the capturing antibody. The extract of the sample of faeces to be tested was applied and detected by the chicken-anti-cestode-lgG which came from hybrid chicken and was cleaned by ammonium sulphate precipitation.A rabbit-anti-chicken-antibody (Sigma) marked with horseradish-peroxidase (POD) served as secondary antibody and converted the substratum (ABTS).The microscopic feacal analysis was used by way of comparison as second method of testing to proof intestinal parasites.The verification of intestinal parasites by microscopic feacal analysis was done with 319 samples, a selecion of 197 samples of feaces was tested with the coproantigen-ELISA.This developed test managed to proof the moniezia-antigen with a total sensitibity of 4 ng protein AG/ml in PBS and 40 ng protein AG/ml in the sample of faeces. The relative sensitivity were determined 80%, with 18% which were wrong doubtful and 2% wrong negativ.This test can only be specified by delimination from other gastrointestinal worms. With samples of feaces that prooved negative to Moniezia, but were afflicted with eggs of trematodes as well as with eggs of nematodes, no influence on the rate of extintion could be monitored.By simplyfiing the method of testing with the result of reducing costs, this method seems to be an alternative to the classical microscopic feacal analyses with the advantage of a higher sensitivity.