Publikationsdatenbank

Degradation of Cry1Ab protein from genetically modified maize in the bovine gastrointestinal tract (2005)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Lutz, Bodo

Wiedemann, Steffi

Einspanier, Ralf

Mayer, Johann

Albrecht, Christiane

Quelle

Journal of agricultural and food chemistry : a publication of the American Chemical Society

Bandzählung: 53

Heftzählung: 5

Seiten: 1453 – 1456

ISSN: 0021-8561

Sprache

Englisch

Verweise

Pubmed: 15740023

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Immunoblotting assays using commercial antibodies were established to investigate the unexpected persistence of the immunoactive Cry1Ab protein in the bovine gastrointestinal tract (GIT) previously suggested by enzyme-linked immunosorbent assay (ELISA). Samples of two different feeding experiments in cattle were analyzed with both ELISA and immunoblotting methods. Whereas results obtained by ELISA suggested that the concentration of the Cry1Ab protein increased during the GIT passage, the immunoblotting assays revealed a significant degradation of the protein in the bovine GIT. Samples showing a positive signal in the ELISA consisted of fragmented Cry1Ab protein of approximately 17 and 34 kDa size. Two independent sets of gastrointestinal samples revealed the apparent discrepancy between the results obtained by ELISA and immunoblotting, suggesting that the antibody used in the ELISA reacts with fragmented yet immunoactive epitopes of the Cry1Ab protein. It was concluded that Cry1Ab protein is degraded during digestion in cattle. To avoid misinterpretation, samples tested positive for Cry1Ab protein by ELISA should be reassessed by another technique.