Publikationsdatenbank

Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC):
a new in vitro model for safety assessment of recombinant food compounds (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Bondzio, Angelika

Stumpff, Friederike

Schön, Jennifer

Martens, Holger

Einspanier, Ralf

Quelle

Food and chemical toxicology

Bandzählung: 46

Heftzählung: 6

Seiten: 1976 – 1984

ISSN: 0278-6915

Sprache

Englisch

Verweise

DOI: 10.1016/j.fct.2008.01.038

Pubmed: 18325653

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The growing use of genetically modified crops necessitates viable screening methods for safety evaluation of recombinant feed, particularly for ruminants. A new sheep rumen epithelial cell culture is introduced as an in vitro cell system for safety evaluation especially focussing on feed and food compounds. We used lactate dehydrogenase (LDH) release, WST-1 conversion, ATP content and caspase 3/7 activity to evaluate cytotoxicity of Cry1Ab, one of the newly expressed Bt-proteins in transgene maize. The results were compared to the effects of valinomycin, a potassium ionophore known to induce cytotoxic effects on a wide range of cells. Whereas no toxicity of Cry1Ab was observed in short as well as in long term experiments, even at non-physiological high concentrations, exposure to valinomycin induced apoptosis and a significant response of all viability parameters after a number of hours. The ATP content and the WST-1 conversion reflecting the energy metabolism of the cells appear to be more sensitive indicators of valinomycin toxicity than the LDH release, a parameter which reflects the membrane integrity. This study presents an in vitro model system, that may be useful as a supplementary tool in toxicity screening before testing substances on animals in vivo.