Fachbereich Veterinärmedizin



    Differential gene regulation under altered gravity conditions in follicular thyroid cancer cells:
    relationship between the extracellular matrix and the cytoskeleton (2011)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Ulbrich, Claudia
    Pietsch, Jessica
    Grosse, Jirka
    Wehland, Markus
    Schulz, Herbert
    Saar, Katrin
    Hübner, Norbert
    Hauslage, Jens
    Hemmersbach, Ruth
    Braun, Markus
    van Loon, Jack
    Vagt, Nicole
    Egli, Marcel
    Richter, Peter
    Einspanier, Ralf
    Sharbati, Soroush
    Baltz, Theo
    Infanger, Manfred
    Ma, Xiao
    Grimm, Daniela
    Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology; 28(2) — S. 185–198
    ISSN: 1015-8987
    DOI: 10.1159/000331730
    Pubmed: 21865726
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62225

    Abstract / Zusammenfassung

    Extracellular matrix proteins, adhesion molecules, and cytoskeletal proteins form a dynamic network interacting with signalling molecules as an adaptive response to altered gravity. An important issue is the exact differentiation between real microgravity responses of the cells or cellular reactions to hypergravity and/or vibrations. To determine the effects of real microgravity on human cells, we used four DLR parabolic flight campaigns and focused on the effects of short-term microgravity (22 s), hypergravity (1.8 g), and vibrations on ML-1 thyroid cancer cells. No signs of apoptosis or necrosis were detectable. Gene array analysis revealed 2,430 significantly changed transcripts. After 22 s microgravity, the F-actin and cytokeratin cytoskeleton was altered, and ACTB and KRT80 mRNAs were significantly upregulated after the first and thirty-first parabolas. The COL4A5 mRNA was downregulated under microgravity, whereas OPN and FN were significantly upregulated. Hypergravity and vibrations did not change ACTB, KRT-80 or COL4A5 mRNA. MTSS1 and LIMA1 mRNAs were downregulated/slightly upregulated under microgravity, upregulated in hypergravity and unchanged by vibrations. These data indicate that the graviresponse of ML-1 cells occurred very early, within the first few seconds. Downregulated MTSS1 and upregulated LIMA1 may be an adaptive mechanism of human cells for stabilizing the cytoskeleton under microgravity conditions.