Fachbereich Veterinärmedizin



    Feline gonads exhibit tissue specific alternative splicing of oestrogen receptor alpha (ESR1) (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Schoen, J
    Sharbati, S
    Ritter, J
    Jewgenow, K
    Reproduction in domestic animals = Zuchthygiene; 47(Suppl. 6) — S. 30–34
    ISSN: 0936-6768
    DOI: 10.1111/rda.12065
    Pubmed: 23279460
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
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    Abstract / Zusammenfassung

    Male felids frequently show teratospermia. At least in the domestic cat model, teratospermia is accompanied by impaired regulation of testicular apoptosis. We hypothesize that this phenomenon is caused by dysregulations in oestrogen signalling pathways. Both classical oestrogen receptors (ESR1 and 2) are expressed in species and/or tissue-specific manners and display different variants, inter alia, caused by alternative splicing. In vitro studies showed that exon deleted transcripts are translated into proteins and that some of the variants modify the effects of the full-length ERs. It has been proposed that some of the functional and morphological dysregulations, for example, during spermatogenesis, could directly derive from this phenomenon. In the present basic study, we investigated the expression pattern of ESR1 splicing variants in the gonads of domestic cats. Testicular, epididymal as well as ovarian tissue samples were collected from routine castrations. ESR1 variants were detected by means of RT-PCR using primers spanning one to three exons. We detected the variants Δ4 and Δ7 in all tissue samples investigated. Additionally, the testicular parenchyma expressed the variant Δ6 and double exon deletions of ESR1 (Δ4/6 and Δ6/7). Using an antiserum recognizing all previously identified ESR1 splicing variants, we revealed ESR1 proteins being expressed in nearly all cells of the testicular and ovarian parenchyma. ESR1 Δ6 protein, however, detected by an antiserum specifically raised against the Δ6 variant, was predominantly located in Sertoli cells. As the exon deletion variants are significantly expressed and show a distinct expression pattern, they could specifically modulate the cellular responsiveness to hormonal stimuli within the gonads.