Fachbereich Veterinärmedizin



    Short-term weightlessness produced by parabolic flight maneuvers altered gene expression patterns in human endothelial cells (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Grosse, Jirka
    Wehland, Markus
    Pietsch, Jessica
    Ma, Xiao
    Ulbrich, Claudia
    Schulz, Herbert
    Saar, Katrin
    Hübner, Norbert
    Hauslage, Jens
    Hemmersbach, Ruth
    Braun, Markus
    van Loon, Jack
    Vagt, Nicole
    Infanger, Manfred
    Eilles, Christoph
    Egli, Marcel
    Richter, Peter
    Baltz, Theo
    Einspanier, Ralf
    Sharbati, Soroush
    Grimm, Daniela
    The FASEB journal : official publ. of the Federation of American Societies for Experimental Biology; 26(2) — S. 639–655
    ISSN: 0892-6638
    DOI: 10.1096/fj.11-194886
    Pubmed: 22024737
    Institut für Veterinär-Biochemie

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    Abstract / Zusammenfassung

    This study focused on the effects of short-term microgravity (22 s) on the gene expression and morphology of endothelial cells (ECs) and evaluated gravisensitive signaling elements. ECs were investigated during four German Space Agency (Deutsches Zentrum für Luft- und Raumfahrt) parabolic flight campaigns. Hoechst 33342 and acridine orange/ethidium bromide staining showed no signs of cell death in ECs after 31 parabolas (P31). Gene array analysis revealed 320 significantly regulated genes after the first parabola (P1) and P31. COL4A5, COL8A1, ITGA6, ITGA10, and ITGB3 mRNAs were down-regulated after P1. EDN1 and TNFRSF12A mRNAs were up-regulated. ADAM19, CARD8, CD40, GSN, PRKCA (all down-regulated after P1), and PRKAA1 (AMPKα1) mRNAs (up-regulated) provide a very early protective mechanism of cell survival induced by 22 s microgravity. The ABL2 gene was significantly up-regulated after P1 and P31, TUBB was slightly induced, but ACTA2 and VIM mRNAs were not changed. β-Tubulin immunofluorescence revealed a cytoplasmic rearrangement. Vibration had no effect. Hypergravity reduced CARD8, NOS3, VASH1, SERPINH1 (all P1), CAV2, ADAM19, TNFRSF12A, CD40, and ITGA6 (P31) mRNAs. These data suggest that microgravity alters the gene expression patterns and the cytoskeleton of ECs very early. Several gravisensitive signaling elements, such as AMPKα1 and integrins, are involved in the reaction of ECs to altered gravity.